Question

BIOCHEM QUESTION:

When solving a protein structure using X-ray crystallography, the crystallographer generates a 3-D grid called an electron density map based on the observed diffraction pattern. The higher the resolution, the more detailed the electron density map and therefore the easier it is to identify what atoms (and therefore what amino acids) are in a given position. Based on the three choices below, in which of the following groups could the two of amino acids be the easiest to differentiate regardless of resolution?

I.	Leucine vs. Isoleucine
II.	Phenylalanine vs. Alanine
III.	Glutamate vs. Glutamic acid

Answer 1

when solving protein structele uring X-ray crystallography the crystallographer generates a 3D gaid bared ou diffrach on pattera, Heigher the resolution, more detailed is the electron density mall and thus easier to identify atoms (ie amino acids. Hence, we ould easily differentiat Phenylalanine ve Alanine regardless of the resolution There are hydrophobic groups! nosmally buried inside the protein