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why is a mitosis inducing agent such as phytohemagglutinin important for generating a karyotype?

why is a mitosis inducing agent such as phytohemagglutinin important for generating a karyotype?

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This is because a karyotype is prepared using chromosomes present in the metaphase stage of mitosis. At this stage, chromosomes are maximally condensed and their morphology can be studied very easily. The morphology includes shape and size of the chromosomes.

So, any mitosis inducing agent will cause the cell to under mitosis and than the photograph of the cells present at metaphase of mitosis can be taken. These chromosomes are than cut, arranged into homologous pairs and a karyotype is formed.

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