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Note that the same abstract questions can be slightly changed and lead to a completely different...


Note that the same abstract questions can be slightly changed and lead to a completely different answer - this is fair game f
You wish to confirm that the resistance allele is dominant. Which of the following could be used to perform this task? A Plan
Note that the same abstract questions can be slightly changed and lead to a completely different answer - this is fair game for any test. The objective is not to memorize questions and answers but to understand the concept. PLOS ONE 8(7) 2013 Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps 10 in a Chinese Soybean Cultivar Jiging Zhang, Changjian Xia, Canxing Duan, Suli Sun, Xiaoming Wang, Xiaofei Wu Zhendong Zhu Resistance to Phytophthora sojae isolate PSMC1 was evaluated in 102 F2:3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar Williams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd 15-24/25 and Sattwd 15-47 flanked the resistance gene at a distance of 0.5 CM and 0.8 CM, respectively. Two cosegregating markers, Sattwd 15-28 and Sattwd 15-32, were also screened in this region. This is the first Rps resistance gene mapped on chromosome 17, which is designated as Rps 10. Eight putative genes were found in the mapped region between markers Sattwd 15-24/25 and Sattwd 15- 47. Among them, two candidate genes encoding serine/threonine (Ser/Thr) protein kinases in Wandou 15 and Williams were identified and sequenced. And the differences in genomic sequence and the putative amino acid sequence, respectively, were identified within each candidate gene between Wandou 15 and Williams. This novel gene Rps 10 and the linked markers should be useful in developing soybean cultivars with durable resistance to P. sojae. Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar Zhang J, Xia C, Duan C, Sun S, Wang X, et al. (2013) Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar. PLoS ONE 8(7): e69799. doi: 10.1371/journal.pone.0069799
You wish to confirm that the resistance allele is dominant. Which of the following could be used to perform this task? A Plant F1 seed in Phytophthora sojae contaminated soil, counting uninfected plants B Plant F2 seed in Phytophthora sojae contaminated soil, counting uninfected plants C Test the parents with the molecular marker Sattwd15-24/25 D Test the progeny of a testcross of Williams 350 x the susceptible line APX", counting uninfected plants E Both A and B or You suspect that the resistance allele is recessive. Which of the following could be used to perform this task? A Plant F1 seed in Phytophthora sojae contaminated soil, counting uninfected plants B Plant F2 seed in Phytophthora sojae contaminated soil, counting uninfected plants C Test the parents with the molecular marker Sattwd 15-24/25 D Test the progeny of a testeross of williams 350 x the susceptible line "APX", counting uninfected plants E Both A and B You cross two F2 progeny. A total of 47 of the 92 seed are resistant to PSMCI: what were the genotypes of the two F2-derived parents (R - resistant allele, r = susceptible allele)? A RRX IT BRr x Rr C Rrx Drexer E Unable to determine or You cross two F2 progeny. A total of 47 of the 192 seed are resistant to PSMC1: what were the genotypes of the two F2 derived parents (R-resistant allele,r-susceptible allele)? A RRX IT B Rrx Rr C Rrxrr Drrrr E Unable to determine which of the following would contradict the research reported in this abstract? A Finding a closely linked marker to the gene for resistance on chromosome 12 B Using the same parents to determine that resistance to F. Solani is also on chr. 17 C Discovering a quantitative trait loci in the same region on chromosome 17 D Crossing Wandou 15 with a different susceptible line and being unable to map to any linkage group E All of the above or Which of the following would support the research reported in this abstract? A Finding a closely linked marker to the gene for resistance on chromosome 12 B Using the same parents to determine that resistance to F. Solani is also on chr. 17 C Discovering a quantitative trait loci in the same region on chromosome 17 D Crossing Wandou 15 with a different susceptible line and being unable to map to any linkage group E None of the above 206
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