Question

Help needed on complementation testing of yeast.

Eight uncoordinated mutants are recovered in a mutagenesis screen. A complementation test is carried out and the results are displayed below. You also determine that unc-2 in your screen is an allele of a previously described mutant, unc-247. Which other mutants recovered in this screen are most likely also alleles of unc-247? unc-unc-unc-uncunc-unc-uncUnc- 8 unc- unc- UNC WT WT WT UNC UNC unc- unc- UNC | WT | WT |WT unc- UNC WT WT 6 UNC UNC unc- unc- UNC 8 unc-1 unc-3 unc-4 unc-5unc-6 unc-7 unc-8

Answer 1

When two mutants are crossed and the resultant F1 progeny shows WT phenotype, it means that both the mutations are located in different genes. i.e. they are non-allelic.

When two mutants are crossed and the resultant F1 progeny shows mutant phenotype, it means that both the mutations are located in the same gene. i.e. they are allelic to each other.

1. Complementation groups
i. unc-1 = unc-4 = unc-5
ii. unc-2 = unc-6
iii. unc-3 = unc-7 = unc-8
unc-6 is an allele of unc-247

2. Complementation groups
i. unc-1
ii. unc-2 = unc-7
iii. unc-3 = unc-4 = unc-6
iv. unc-5
v. unc-8

5. Option B is correct.
In a suppressor screen, a mutant organism is mutagenized to obtain suppressors of its phenotype.