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You are given a fresh meat sample containing 5.6 times 10^5 bacteria/g. Diagram how you would...
6) How many bacteria would be in 1 ml of the original sample if the 108...
6) How many bacteria would be in 1 ml of the original sample if the 108 dilution has 200 colonies? 7) What criteria must your plates meet in order to be considered "countable" ? How many bacteria per milliliter of sample would be "acceptable" to the FDA? - 86 -
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to...
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to a bottle containing 99ml of sterile water. You then take 0.1 ml from this dilution bottle and transfer to a second bottle with 0.9 ml of sterile water. Finally, you transfer 1 ml to a Petri dish with nutrient agar. How many colonies would you expect to find on this plate? Would it be countable?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete...
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold...
1. You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil? 2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells. a) After 5...
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial...
You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies. a) What was the dilution factor? b) How many bacteria were present in the soil?
You have three pure cultures of bacteria. One is Gram positive bacteria and the other two...
You have three pure cultures of bacteria. One is Gram positive bacteria and the other two are Gram negative bacteria but you have mixed up the labels. You know that one of the Enteric bacteria can ferment glucose and produce ethanol and CO_2. The other can ferment glucose and produce lactic acid. Describe the general procedure you could perform to determine of the three cultures which one is which? A one gram sample of hamburger is diluted to 10^-4 in...
Suppose you would like to determine the number of bacteria in a culture via an aerobic...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
You have a tube with 5ml of infected blood that has a concentration of bacteria at...
You have a tube with 5ml of infected blood that has a concentration of bacteria at 10(8) cells per ml. You wish to make a serial dilution of the blood so when you take 100 ul of the last dilution and spread it on a Petri dish you will have between 30-300 colonies growing on the plate. You have plenty of empty 1.5 ml microfuge tubes, a bottle of sterile water, a p1000 pipettor (100-1000ul) and tips, for the dilutions,...
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
6) How many bacteria would be in 1 ml of the original sample if the 108 dilution has 200 colonies? 7) What criteria must your plates meet in order to be considered "countable" ? How many bacteria per milliliter of sample would be "acceptable" to the FDA? - 86 -
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
You have three pure cultures of bacteria. One is Gram positive bacteria and the other two are Gram negative bacteria but you have mixed up the labels. You know that one of the Enteric bacteria can ferment glucose and produce ethanol and CO_2. The other can ferment glucose and produce lactic acid. Describe the general procedure you could perform to determine of the three cultures which one is which? A one gram sample of hamburger is diluted to 10^-4 in...
Suppose you would like to determine the number of bacteria in a culture via an aerobic plate count. Explain (with pictures) how you would set up a dilution series (104, 106, 10) using the fewest number of dilution blanks. Draw and label your tubes and plates with each dilution. Show all volumes including transfers, amounts in tubes, and amounts plated 1. You want to set up a 1:10 dilution series, so that you can plate a 10-1, 102, and a...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
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