Question

How would you determine outer membrane localization and how would it differ from inner plasma membrane?

Hydropathy Plot: Kyte & Doolittle scale +2 +1 0 CD -1 -2 3 11 residue window 50 100 150 200 250 300 ZhoA residue position

Answer 1

Hydropathy plot- Hydrophathy plot help to visualization of hydrophobicity of peptide sequence,by this kind of plot we could easily distinguish between hydrophobic and hydrophilic amino acid from peptide sequence.By this graph we could conclude how many times any peptide crossing to plasma membrane.

scale on hydropathy plot --On X-axis it indicating number of amino acid.while on Y-axis it indicating values from negative to positive.mid point of value on Y-axis is zero.less than zero value which indicated by negative sign is use for hydrophobic amino acids while positive values indicate hydrophilic amino acids.

For crossing to plasma membrane once, 20 hydrophobic amino acids are needed.If in plot 20 hydrophobic amino acids continuously, it means that peptide crossing plasma membrane once to plasma membrane.

if more amino acids present in positive values it means those amino acids are hydrophilic and present outside to plasma membrane.

Cx 10