Question

QUESTION 3 Replication Now DNA Origin of replication DOC Replication fork B CON RO Unreplicated DNA Prokaryotic DNA The above diagram shows DNA replication in bacterial cells. An antibody specific only to DNA polymerase I was added to DNA undergoing replication in bacterial cells. Which of the following statements are CORRECT? a. There would be a higher concentration of antibodies on the leading strands of replication foris A and B and a lower concentration of antibodies on the lagging strands of replication forks A and B. b. There would be a higher concentration of antibodies on the lagging strands of replication forks A and B and a lower collcentration of antibodies on the leading strands of replication forks A and B. There would be a higher concentration of antibody on the lagging strand of replication fork and a lower concentration of antibody on the lagging strand of replication fork B. d. There would be a higher concentration of antibody on the leading strand of replication fork A and a lower concentration of antibody on the leading strand of replication fork B. e. No antibodies would bind to bacterial DNA since DNA polymerase is not used during DNA replication

Answer 1

Correct answer is option b.

As we know that the function of DNA polymerase 1 during bacterial replication is to join the nicks between the okazaki fragments on the lagging strand. At first RnaseH removes the RNA primers that are synthesized by primase,from the lagging strand then the DNA polymerase1 fills up those gaps created by removal of RNA primers by adding complementary nucleotides between the okazaki fragments to form a continuous DNA strand.

So definitely DNA polymerase 1 specific antibody will have more concentration on the lagging strands but not leading strands as it does not have any function on leading strand.