Question

1st attempt When using tandem mass spectrometry for peptide sequence determination, what is the input into the second mass spectrometer from the collision chamber? Choose one: A. Peptides from a select range of masses B. Tryptic fragments of the protein of interest C. Isolated and fragmented peptides D. Whole protein

Answer 1

Tandem mass spectrometry or MS/MS or MS2, is an instrumental technique to analyze protein or peptide sequence. In MS/MS two or more mass spectrometers are coupled together and use an additional reaction step that increases their abilities to analyse chemical samples

The following scheme explains how Tandem MS works.

1. Samples are first ionized by ESI, MALDI, EI, etc. to generate a mixture of ions,

2. The ions are then separated by their specific mass-to-charge ratio (m/z) in MS1

3. The fragmented peptides and then injected to MS2 to generate a product (ions) for detection.

4. MS2 again separates the fragments by their m/z-ratio and detects them.

So the correct option is option c. isolated and fragmented peptides.

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