Question

design a forward and reverse PCR primer based on underlined sequence in the following DNA sequence. 3' AAGCCAGGCCCTGGAGT......................TGGACTCGTGGGTGTG.....5'

What does "PCR stand for and briefly describe PCR program

Answer 1

Forward primer
5' UUCGGUCCGGGACCUCA 3'

Reverse primer
5' UGGACUCGUGGGUGUG 3'

PCR stands for Polymerase Chain Reaction and the equipment used is Polymerase Chain Reactor

Program of PCR
After addition of chemicals in a proper proportion to PCR tubes the PCR can be completed in following steps

Step. 1 Denaturation
In this step the double standard DNA is separated into two strands at 94 degree Centigrade

Step. 2 Annealing
The primer will attach to the single stranded DNA

Step. 3 Extension
In this reaction the Taq DNA polymerase will attach to the single stranded DNA at Primer attached site and start polymerization of DNA