
Enzyme activity will be high when substrate concentration is equal to KM b. below KM ...
When substrate concentration is much less than KM, the rate of catalysis is almost equal to A) Kd B)Vmax / KM C) kcat D) None of the above E) Vmax ((A-E) are answer choices)
What is the consequence of a high Km value of an enzyme? High concentration of the substrate is needed for the maximal enzyme activity. The enzyme is present in a large concentration in the cell. Moderate concentration of the substrate will be enough for maximal enzyme activity. This enzyme has high maximal velocity.
What is substrate concentration, expressed as a multiple of Km, when an enzyme reaction is observed to have an initial rate Vo = 0.75 Vmax. Select one: O a. [S] = 0.33 x km O b. [S] = 0.25 km O c. [S] = 0.75 x Km O d. [S] = 0.3 x km O e. [S] = 0.5 x km Check Next page ime Jump to...
Which of the following statements are corrects? When the substrate concentration is equal to KM, the reaction rate is half of its maximal value The value of KM is proportional to the concentration of the ES complex Inhibitors are molecules capable of decreasing the enzyme activity Uncompetitive inhibitors have no effect on Vmax fill in the blanks The respiratory chain is a set of_______complexes whose role is to transport _______ from reduced co-factors to dioxygen. The (entry from previous blank)...
3. Why is an allosteric enzyme more sensitive to substrate concentration around Km values than a Michaelis-Menten enzyme with the same Vmax? 4. Explain how pH and temperature influence enzyme activity. ( A Lineweaver-Burk (double reciprocal) plot was used to compare the effects of three different reversible inhibitors (A, B and C) on an enzyme. The plot of 1/V vs 1/[S] for the enzyme with no inhibitor is shown in a solid black line. The plot of 1/V vs 1/[S]...
At what substrate concentration would an enzyme with a kcat of 30.0 sec-1 and a Km of 0.0030 M have an initial velocity that is 25% of the maximum velocity? a. 0.0010M b 0.0090 M c. 0.0015 M d. 0.0060 M e.Cannot be determined
At what substrate concentration would an enzyme with a kcat of 30.0 sec-1 and a Km of 0.0030 M have an initial velocity that is 25% of the maximum velocity? a. 0.0010M b 0.0090 M c. 0.0015 M d. 0.0060 M e.Cannot be determined
6. An enzyme with a km of 0.06mmol/L hydrolyzed a substrate of a concentration 0.03 mmol/L. The initial velocity ws 0.0015 mmol/L.min!. Calculate the substrate concentration which gives an initial velocity of 0.003 mmol/L. min-1 7. Urease hydrolyzed urea at [s]=0.03mmol/L with a km of 0.06 mmol/L. The initial velocity observed was 1.5X10-3 mmol/L.min Calculate the initial velocity of the enzyme reaction when using [s]=0.12mmol/L
Can
you infinitely infrease the substrate concentration to increase
enzyme activity in a closed system such as a batch culture?
Can you infinitely increase the substrate concentration to increase enzyme activity in a closed system such as a batch culture? Yes Your answer B NO
If the substrate concentration is varied with a fixed concentration of enzyme, it is observed that at low substrate concentrations that overall order while at high substrate concentrations, the reaction will be overall order. Select one: a. first; second b. first; third order c. first; zero d. second; second e. second; pseudo first order