Question

QUESTION 8 Calculate the extinction coefficient in (mg/mL) cm from the following data. It is suggested that you use Excel to do this. Give your answer in 3 significant figures. Assume the path length is 1 cm. (You may want to look at the write-up for LAB 19 -1 c (mg/mL) Abs (e 500 nm) 0.001 0.245 0.002 0.490 0.003 0.735 0.004 0.980 0.005 1.100 0.006 1.200 0.007 1.250

Answer 1

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D E F G H J， K ། 0.245 0.49 Chart Title 0.735 C(mg/mL) Abs(@500 nm) 0.001 0.002 0.003 0.004 0,005 0.006 0.007 0.98 1.1 1.2 1.25 y= 0 1714x + 0 1714 ༢༧ = 0.9454 Axis Title -C(mg/mL) =Abs{@500 nm) Linear (Abs(@500 nm)) 1 2 3 4 5 5 】་ 17 18 slope 0,1714 19 Y-intercept 0.171428571 20 path length=1 cm 21_Molar extinction coefficient =0.1714/1 0.1714L mol-1 Cm-1 23 ༈