How do you calculate the average sizes and numbers of DNA fragments produced by digesting human...
How do you calculate the average sizes and numbers of DNA fragments produced by digesting human genomic DNA with a given restriction enzyme?
Homework Answers
After digestion of DAN by restriction enzymes multiple DNA fragments with variable length is produced. So we can use electrophoresis technique to seprate the DNA strand. In electrophoresis DNA molecules are seprated depends on charge on the molecules. Smaller fragments will move faster than large fragments. Thus based on migration pattern of DNA and by comparing it with DNA ladder . We can estimate different no of DNA band and size of band.
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How do you calculate the average sizes and numbers of DNA
fragments produced by digesting human...
5. If you are digesting a large linear fragment that is 300,000 bp in length. How...
5. If you are digesting a large linear fragment that is 300,000 bp in length. How many fragments would be produced if the DNA was digested by the following enzymes? Assume the fragment sizes are the average sizes calculated for a random DNA sequence. Use the information in the table from Question 3. Show your work. (A) Smal (B) Haelll (C) Noti Table 4.1 Recognition Sequences and Cutting Sites of Selected Restriction Endonucleases Enzyme Alul BamHI Bgli Clal ECORI Haelll...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments were produced of sizes 6 kb and 4 kb. The restriction enzyme HaeIII (H) also produced two fragments of sizes 9 kb and 1 kb. When the two enzymes HaeIII and EcoRI were used together, three fragments were produced of sizes 1 kb, 3 kb, and 6 kb. A 4 kb long cloned genomic probe from this region hybridized to the 3 kb and...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
Human mt DNA has 2 EcoR1 restriction sites •Honey bee mt DNA has 5 restriction sites...
Human mt DNA has 2 EcoR1 restriction sites •Honey bee mt DNA has 5 restriction sites Q: How many bands would you see on a gel after digesting human and honey bee mtDNA with the EcoR1 restriction enzyme? Hint: mtDNA is circular in both humans and honey bees.
A linear DNA molecule was digested with restriction enzyme EcoR I (E) and three fragments were...
A linear DNA molecule was digested with restriction enzyme EcoR I (E) and three fragments were produced of sizes 7.3 kb, 4.0 kb and 3.4 kb. The restriction enzyme BamH I (B) produced three fragments of sizes 7.9 kb, 4.8 kb and 2 kb. When the two enzymes EcoR I and BamH I were used together, five fragments were produced of sizes 6.5kb, 4kb, 2kb, 1.4kb, and 0.8kb. Which of the following is the correct restriction map on this molecule...
1) If a restriction enzyme cuts a circular DNA into five fragments, how many restriction sites...
1) If a restriction enzyme cuts a circular DNA into five fragments, how many restriction sites are there in the DNA? 2) How many molecules of DNA will be present after 6 cycles of PCR, if you started with one double-stranded DNA molecule? CELL BIOLOGY QUESTIONS!! SHOW WORK PLEASE
. Using the figure of the gel below, draw in the DNA bands in the left...
. Using the figure of the gel below,
draw in the DNA bands in the left lane that would appear from the
DNA of the person described in the question above. The right lane
contains a molecular weight standard.
the questions are
When circular DNA is sequenced, the
nucleotide base pairs are numbered starting from an fixed position
on the DNA, all the way around, usually in a clockwise
manner. a DNA molecule that is 3133 base pairs long is...
Conceptual Questions L You are working as a technician in a lab, and your supervisor has...
Conceptual Questions L You are working as a technician in a lab, and your supervisor has asked you to isolate the gene for super sweet corn (ssc/) from-the sample of corn genomic DNA. She gives you the following map, and she also gives you a radioactively labeled probe that is specific for the scl gene. Devise an experimental protocol to isolate the gene from-the genomic DNA, what enzymes would you use to digest the DNA? How would you isolate the...
5. If you are digesting a large linear fragment that is 300,000 bp in length. How many fragments would be produced if the DNA was digested by the following enzymes? Assume the fragment sizes are the average sizes calculated for a random DNA sequence. Use the information in the table from Question 3. Show your work. (A) Smal (B) Haelll (C) Noti Table 4.1 Recognition Sequences and Cutting Sites of Selected Restriction Endonucleases Enzyme Alul BamHI Bgli Clal ECORI Haelll...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
. Using the figure of the gel below,
draw in the DNA bands in the left lane that would appear from the
DNA of the person described in the question above. The right lane
contains a molecular weight standard.
the questions are
When circular DNA is sequenced, the
nucleotide base pairs are numbered starting from an fixed position
on the DNA, all the way around, usually in a clockwise
manner. a DNA molecule that is 3133 base pairs long is...
Conceptual Questions L You are working as a technician in a lab, and your supervisor has asked you to isolate the gene for super sweet corn (ssc/) from-the sample of corn genomic DNA. She gives you the following map, and she also gives you a radioactively labeled probe that is specific for the scl gene. Devise an experimental protocol to isolate the gene from-the genomic DNA, what enzymes would you use to digest the DNA? How would you isolate the...
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