This is a microbiology lab question, anyone willing to help, please do! What is the minimum...
This is a microbiology lab question, anyone willing to help, please do!
What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine, and the antibiotic tetracycline but lacking the essential amino acid isoleucine?
Homework Answers
Minimal Media are those media that contain minimal nutrient for colony growth. Now here recombinant cell grow on the minimal media that contain arginine ,methionine and tetracycline antibiotics but lacking isoleucine means that cell are able to synthesize isoleucine and also contain a tetracycline resistance Gene but do not able to synthesize met and arg.so genotype is:-arg- met-, Ile+, tetR.
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This is a microbiology lab question, anyone willing to help,
please do!
What is the minimum...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
need help with question 2, 4, 6 a.&b. 2. For a (fictitious) haploid fungus, the starting...
need help with question 2, 4, 6 a.&b.
2. For a (fictitious) haploid fungus, the starting point in the biosynthesis of the amino acid arginine is Compound X, which is always present in, and absorbed from the environment. The arginine biosynthetic pathway is: Enzyme B → Compound Y Compound z Enzyme C Compound X Enzyme A arginine A mutant strain of genotype a (lacking enzyme A) is crossed to a mutant strain of genotype c (lacking enzyme C). What proportion...
please help with this question. I am taking microbiology class and I really need help. 1....
please help with this question. I am taking
microbiology class and I really need help.
1. 10pts. Below is a diagram of a cell with two differently sized molecules at much higher concentration inside the cell than outside of the cell. For each of the scenarios listed, describe what would move and where: a) the membrane is permeable to both molecules, b) the membrane is only permeable to the smaller molecule, c) the membrane is impermeable to both molecules. Now...
please answer question 4a-c? What is the recombination frequency between trp and cysX? E) What is...
please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is the recombination frequency between ton and cysX? F) What is the order of the three genes? Indicate the distance between them. 4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str 1+ O+ 2+ y + (that you grew in the absence of lactose) with a lac-F-strain with the genotype str - O+ Z-y! You allow sufficient time for the...
hello everyone can anyone help on this pls? what do i request for ? Unknownidentification Form.pdf page...
hello everyone can anyone help on this pls? what do i
request for ?
Unknownidentification Form.pdf page 2 of 2) a sourch Thu 1:47 PM ome Draw Design Transitions Animations Side Show X A Mixed Unknown identification Form Uno Pontecimentication Lab Sen Fond REQUESTING TEST MEDIA DO NOT TAKE MEDIERON MEDIA BENCHWITHOUT PERMISSION FROM YOUR INSTRUCTOR Becoming to the Media Banca You wengi 5 TEST MEDIATHEQUESTED For Gram Positive Identification For Gram-Negative Identification RNIRE 2 S 14 S UNIQUE CHARACTERISTICS &...
Hello please please help !! Thank you!! Please and thank you soo much!!! Question Completion Status:...
Hello please please help !! Thank you!!
Please and thank you soo
much!!!
Question Completion Status: Question 10: The genetic code consists of 64 triplets of nucleotides (called codons). Each codon (with the exception of the 3 stop codons) encodes for one of the 20 amino acids used in the synthesis of proteins. This produces some redundancy in the code as most amino acids are encoded by more than one codon. One codon, AUG serves two related functions: it signals...
can anyone help me with the last question please i did not do it correctly and...
can anyone help me with the last question please i did not do it
correctly and I am unsure how
IMPROVED NEUBAUER RULING You are preparing to plate yeast that you have been growing overnight in your laboratory and you want to determine the cell concentration of the culture. After diluting a small amount of culture 1:10 and loading your hemocytometer, you observe the grid pictured above with your light microscope. 12. What is the cell concentration (cells/ml) of the...
Food Microbiology Laboratory question, **********Question********** (PLease help me to figure out the answers after reading/ using...
Food Microbiology Laboratory question,
**********Question********** (PLease help me to figure out the
answers after reading/ using following text, thx a lot)
1. Deeply analyse and explain the
result of this lab (results is provide in the following
text)
---------------------
Laboratory: Microbiological analysis of food products
(3M Petrifilm E.coli/Coliform (EC) count
plate)
Used media:3M Petrifilm E.coli/Coliform (EC) count plate
3M EC count plates are designed to identify both E. coli and
other coliforms. It contains crystal violet and bile salt which...
all them please Question 1 (1 point) Given the following information: parental phenotypes in the progeny...
all them please
Question 1 (1 point) Given the following information: parental phenotypes in the progeny are a, b c, and a+, b+, C+, and the double recombinant phenotypes are a+, b, C+ and a, b+, c what is the gene order? A) b--a-- B) a--b-c C) a--C--b ហា Question 5 (1 point) What embryonic event gives rise to calico cats? OA) Y inactivation B) x inactivation C) chromosomal non-disjunction D) meiosis Question 3 (1 point) Two parents without sickle...
can anyone help me to solve these q?please!! Question 1 What is the main reason that...
can anyone help me to solve these q?please!!
Question 1 What is the main reason that cause agency problem to occur between the managers and the shareholders in a company? Question 2 Today, you deposit $15,000 in a bank account that pays 8 percent annual simple interest. How much interest will you earn over the next 6 years? Question 3 You want to have $300,000 in cash for down payment of a property 5 years from today. You expect to...
2 What is the minimum genotype of a recombinant cell that grew on minimal media supplemented with arginine, methionine and the antibiotic tetracycline but lacking the essential amino acid isoleucine 3 How would you determine if your recombinant cells had also acquired the tryptophan gene from the Hart Complete the following table indicating which of the organisms (F and/or Hfr used in this experiment would be expected to grow on the given media. Unless specified, MMD is just minimal medium...
need help with question 2, 4, 6 a.&b.
2. For a (fictitious) haploid fungus, the starting point in the biosynthesis of the amino acid arginine is Compound X, which is always present in, and absorbed from the environment. The arginine biosynthetic pathway is: Enzyme B → Compound Y Compound z Enzyme C Compound X Enzyme A arginine A mutant strain of genotype a (lacking enzyme A) is crossed to a mutant strain of genotype c (lacking enzyme C). What proportion...
please help with this question. I am taking
microbiology class and I really need help.
1. 10pts. Below is a diagram of a cell with two differently sized molecules at much higher concentration inside the cell than outside of the cell. For each of the scenarios listed, describe what would move and where: a) the membrane is permeable to both molecules, b) the membrane is only permeable to the smaller molecule, c) the membrane is impermeable to both molecules. Now...
please answer question 4a-c?
What is the recombination frequency between trp and cysX? E) What is the recombination frequency between ton and cysX? F) What is the order of the three genes? Indicate the distance between them. 4. (3) Suppose that you mate a lac+ Hfr strain with the genotype str 1+ O+ 2+ y + (that you grew in the absence of lactose) with a lac-F-strain with the genotype str - O+ Z-y! You allow sufficient time for the...
hello everyone can anyone help on this pls? what do i
request for ?
Unknownidentification Form.pdf page 2 of 2) a sourch Thu 1:47 PM ome Draw Design Transitions Animations Side Show X A Mixed Unknown identification Form Uno Pontecimentication Lab Sen Fond REQUESTING TEST MEDIA DO NOT TAKE MEDIERON MEDIA BENCHWITHOUT PERMISSION FROM YOUR INSTRUCTOR Becoming to the Media Banca You wengi 5 TEST MEDIATHEQUESTED For Gram Positive Identification For Gram-Negative Identification RNIRE 2 S 14 S UNIQUE CHARACTERISTICS &...
Hello please please help !! Thank you!!
Please and thank you soo
much!!!
Question Completion Status: Question 10: The genetic code consists of 64 triplets of nucleotides (called codons). Each codon (with the exception of the 3 stop codons) encodes for one of the 20 amino acids used in the synthesis of proteins. This produces some redundancy in the code as most amino acids are encoded by more than one codon. One codon, AUG serves two related functions: it signals...
can anyone help me with the last question please i did not do it
correctly and I am unsure how
IMPROVED NEUBAUER RULING You are preparing to plate yeast that you have been growing overnight in your laboratory and you want to determine the cell concentration of the culture. After diluting a small amount of culture 1:10 and loading your hemocytometer, you observe the grid pictured above with your light microscope. 12. What is the cell concentration (cells/ml) of the...
Food Microbiology Laboratory question,
**********Question********** (PLease help me to figure out the
answers after reading/ using following text, thx a lot)
1. Deeply analyse and explain the
result of this lab (results is provide in the following
text)
---------------------
Laboratory: Microbiological analysis of food products
(3M Petrifilm E.coli/Coliform (EC) count
plate)
Used media:3M Petrifilm E.coli/Coliform (EC) count plate
3M EC count plates are designed to identify both E. coli and
other coliforms. It contains crystal violet and bile salt which...
all them please
Question 1 (1 point) Given the following information: parental phenotypes in the progeny are a, b c, and a+, b+, C+, and the double recombinant phenotypes are a+, b, C+ and a, b+, c what is the gene order? A) b--a-- B) a--b-c C) a--C--b ហា Question 5 (1 point) What embryonic event gives rise to calico cats? OA) Y inactivation B) x inactivation C) chromosomal non-disjunction D) meiosis Question 3 (1 point) Two parents without sickle...
can anyone help me to solve these q?please!!
Question 1 What is the main reason that cause agency problem to occur between the managers and the shareholders in a company? Question 2 Today, you deposit $15,000 in a bank account that pays 8 percent annual simple interest. How much interest will you earn over the next 6 years? Question 3 You want to have $300,000 in cash for down payment of a property 5 years from today. You expect to...
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