Question

Prefixes in the Metric system

Factor	Prefix	Symbol	Factor	Prefix	Symbol
1012	tera	T	10-1	deci	d
109	giga	G	10-2	centi	c
106	mega	M	10-3	milli	m
103	kilo	K	10-6	micro	μ
102	hecto	H	10-9	nano	n
10	deka	Da	10-12	pico	p

Please work on your own and show all your work. Each question is worth 2 pts.

1. Express these non-exponentially, and chose the unit and prefix that works best: 2 x 10^-3s; 1.5 x 10^-4l; 4 x 10^-8 g

2. Professor Fuchs has performed a Dual-Luciferase Assay in her lab and it is part of a paper she published (Fuchs et al., 2014). This is quoted from the Methods section: “A total of 2.5 μgof a dual-luciferase plasmid DNA, in which expression of the Renilla protein is cap-mediated and expression of the firefly cistron depends on the HCV IRES (15), was mixed with electro-poration buffer (Teknova). A total of 7.5 × 10⁵ cellswere electroporated in 0.1 mmof electroporation cuvettes (BIORAD) using a BIORAD gene pulser           (120 V, 1.5-mspulse length, 10 pulses, 1.5 ms between pulses). Twenty-four hours after electroporation, luciferase activities were measured using the dual-luciferase kit (Promega).”

Write the bolded items exponentially in the respective base units (g, m, s) and write the number of cells in non-exponential forms.

3. The rate of DNA replication is about 2 kb (kilobases) per minute. How many bases is that per second?

4. Dr. Fitz orders 5.0 mg drug per kg of body weight of a medication for a patient. Premixed syringes contain 500 mg of drug per 10 mL of solution. Your patient weighs 176 lbs. How many milliliters of solution should you administer to the patient? (2.2 lbs = 1 kg)

Answer 1

2 x 10^-3s 2ms

1.5 x 10^-4l 150µl

4 x 10^-8 g 40 ng

2.5 μg 2.5x10-6g

7.5 × 10⁵ cells 750000 cells

0.1 mm 0.1x10-3

1.5-ms 1.5x10-3

2000pb----------------60seconds

X-----------------------1SECOND

33.3333pb/s

The patient weights 176lbs which is 176/2.2 kilos so...80 kilos.

if he uses 5mg -----------------1kg

X----------------------80kg

X is equal to 400mg

each syringe contains 500mg/10ml so

500mg------------------ 10ml

400mg ---------------X

X is 8ml.

final answer: he shoud administer 8ml of the solution