DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values...
DNA quantitation. You measure the absorption spectrum for a sample of DNA and obtain the values listed below. The DNA had been diluted 10 fold. Remember that a solution of DNA that is 50μg/ml (or 50ng/μl) has an absorbance of 1.0 at a wavelength of 260 nm. What is the concentration of the DNA in this sample (In this question DO NOT ignore the problem of dust in the sample and give answer in ng/ul).
A260 = 0.80 A280 = 0.40 A320 = .05 A230 = 0.40
Homework Answers
Concentration of DNA= (A260-A320) X dilution factor X 50μg/ml
= (0.80-0.05) X 10 X 50 =375 μg/ml
The DNA concentration of the sample is 375 μg/ml.
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DNA quantitation. You measure the absorption spectrum for a
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After purification of your favorite plasmid you use a
spectrophotometer to find the concentration of your DNA and observe
the graph below. Resketch by hand this graph on your answer page
with exact axis labels and scales. Redraw the red line. Draw, in
your estimation, the absorption spectrum of what is expected of a
good DNA prep.
OD o X Load your sample and press the measure button. Blank Re-Blank Sample ID: Pedestal ort pectra ple volume Type: DNA 50.00...
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