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An interrupted mating experiment was performed, using four different Hfr strains (A, B, C, and D)....

An interrupted mating experiment was performed, using four different Hfr strains (A, B, C, and D). The time in minutes for each of the genes is shown in the table below. How long would you expect the time delay to be between his and lip in an interrupted mating experiment?

Genetic Marker

Hfr

his

lac

leu

lip

pheS

pro

pyrD

terC

tonA

trp

A

-

20

-

11

-

-

3

-

-

-

B

-

-

18

-

-

-

31

-

20

25

C

-

13

-

22

-

6

-

2

-

-

D

19

-

-

-

12

4

-

8

-

-

Select one:

a. 32 minutes

b. 37 minutes

c. 8 minutes

d. 31 minutes

e. 35 minutes

Please explain and or show work.

Thank you.

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Answer #1

Lip and His are present in strains A, D and C, so we will use these three strains to determine the final sequence of the genes. I am attaching a picture to show the work. (Note: the time written on the arrows is the delay between genes to be transferred. For example, in strain A, after pyrD has been transferred, lip takes 8 minutes to be transferred next.

From the above calculations, the delay between his and lip = 7+4+4+7+9 = 31 minutes.

Option (d) is correct.

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