Question

Sample (gamma globulin)	volume (microliter)	water (microliter)	dye (microliter)	absorbance (A595)
100(microgram/ml)	50	550	400	.037
100(microgram/ml)	100	500	400	.076
100(microgram/ml)	150	450	400	.103
100(microgram/ml)	200	400	400	.133
100(microgram/ml)	250	350	400	.156
100(microgram/ml)	300	300	400	.185
100(microgram/ml)	400	200	400	.219
Unknown	25 (microgram/ml)	575	400	.075
Unknown	50 (microgram/ml)	550	400	.12
Unknown	100 (microgram/ml	500	400	.185

1) what is the amount of (in micrograms) of gamma globulin in the diluted unknown samples? Show work and all units please for all three dilutions (last three rows)

2)For each of the diluted, find the undiluted concentration. Average will be the undiluted concentration of the unknown sample. Units are micrograms/ml

Answer 1