Question

D. CHROMATOGRAPHY Chromatography is a general technique that is used to separate various materials. In this case, students can use the unique chemistry of the amino acid to determine its migration in a specific solvent. The solutions of amino acids can be applied to the place and allowed to dry. The plate is placed in the solvent, and capillary action will carry the amino acids upward along the plate. The side chains, or R groups, can be characterized by their chemical proper ties as nonpolar, polar uncharged, or polar charged. Those amino acids that are the most nonpolar will travel farther on the plate, while those that are charged and polar will stay near the bottom of the plate. This is because the plate is polar and more strongly attracts polar molecules, slowing their progress up the plate. In addition, the same amino acid will travel the same distance no matter what lane it is in. In this way, a mixture of amino acids may be separated and their identity matched with a standard. 1. Suppose you had the following result (below). Answer the following questions. a. Which amino acid tested is most polar? b. Look up the structure of the amino acids. Explain why it makes sense that the amino acid you listed is more polar than the others. c. What amino acids are in the fruit juice? ------ - --- -- Isoleucine Serine Glutamic acid Fruit juice

Answer 1

Ans - 1(a) amino distance Glutamic acid acid because than others. és most polar it travel lesser Ang L(b) Coo HN-C-H (12 Cool- HH-C-H Cool 1₂N-C-H 11e-CH3 CH₂ - CH3 isoleucin and. c72 H-C-oh Cool glutamic Senne qutamic acid és most polar has polar side chain with Because it has negative parel lesser distance. because it Cool (carboxyl group). charge et Serine is uncharged but polar amino acid so it have more distance than glutamic acid 9 solencin es nonpolar molecule so it trand highest distance, +(0) Glutamic acid is present in the muit Juice because glutamic acid and fruit juice travel the same Any distance.