A throat culture grows normal oropharyngeal microbiota. The coagulase test for the organism is positive. What is the genus and species of the organism?
1. Staphylococcus
aureus
2. Streptococcus agalactiae
3. Streptococcus pyogenes
4. Staphylococcus epidermidis
1. staphylococcus aureus
pharynx is called throat : these contains 3 parts oropharynx and nasopharynx, laryngopharynx or hypopharynx .
oropharyngial microbiota is major reservoir for infections.
aureus produce two types of coagulases. that is free coagulase and bound coagulase.
coagulase test organism is Positive ....in this test produces coagulase enzyme with catalyzes the conversion of soluble fibrinogen in plasma to insoluble fibrin.
A throat culture grows normal oropharyngeal microbiota. The coagulase test for the organism is positive. What...
What is the catalase test? Coagulase test? What does it mean to be: Catalase + Catalase - Coagulase + Coagulase - When working with an unknown that may be Staphylococcus (but you are not sure), how can the two tests help? Give step by step detail of how to determine you are dealing with Staphylococcus and not an organism such as Streptococcus (next week topic). Give step by step details on how to determine you are dealing with: S. epidermidis...
Case Study
#1
Clinical
history: An HIV positive male presented in clinic with
confusion and disorientation. He had a fever 38.5°C and
photophobia. His CD4 T cell count was 80/ul. An MRI and lumbar
puncture were performed. The MRI showed various small lesions
within the brain. CSF analysis indicated 32 White Blood Cells/ul
with 89% lymphocytes, and 6% monocytes, glucose of 22mg/dl, and
protein of 89mg/dl. Gram stain showed yeast and India ink negative
stain showed a thick capsule.
Image...
Which of these organisms are normal flora of the throat and are associated with dental caries, brain abscesses, and the case study presented here? Streptococcus pyogenes Streptococcus viridans Streptococcus pneumoniae Peptostreptococcus species Staphylococcus aureus
Microbiology Matching: a. Mannitol b. Coagulase c. Catalase d. Novobiocin e. Camp f. Bacitracin g. Optochin 1. ___ Genus Streptococcus identified by negative reaction 2. ___ S. aureus is identified by its positive reaction 3. ___ S. saprophyticus is identified by its resistance 4. ___ S. epidermidis is identified by its negative reaction 5. ___ S. pyogenes is identified by sensitivity 6. ___ S. pneumoniae is identified by its sensitivity. 7. ___ S. agalactias is identified by its positive reaction
1. The Gram reaction; Gram Positive or Gram Negative 2. The shape of the cells; bacillus, coccus, spiral 3. The typical arrangement of the cells; single, pairs, clusters, chains etc. Shape of cells Arrangement of cells Name of organism Gram Reaction Bacillus cereus Enterobacter aerogenes Enterococcus durans Escherichia coli Micrococcus luteus Senatra marcescens Staphylococcus epidermidis streptococcus Salivarius Klebsiella pmeumonia Salmonella entertidis Shigella Sonnei Staphylococcus aureus Streptococcus pyogenes *Hint: The organisms used in the Lab. are listed in the Safety Guidelines...
All of the following are characteristic of Staphylococcus aureus EXCEPT 1- produces coagulase 2- ferments mannitol 3- grows on an MSA plate (i.e., halotolerant) 4- grows in chains You have isolated gram-positive cocci from a throat culture that you cannot identify as staphylococci or streptococci. A test for one enzyme can be used to distinguish these bacteria quickly. What is the enzyme? 1- dogalase 2- catalase 3- lactamase A candle jar is used to culture Streptococci because they 1- grow...
Laboratory & Exercises 1. You will not be physically performing these activities. However, you will be responsible for knowing how to perform and interpret them. View the following video clips: • SE Broth & Bile Esculin • CAMP Test 2. Review how to interpret the following tests by completing the chart: Positive Result Negative Result Gram Stain for cocci and bacilli purple Schaeffer-Fulton Stain for Endospores Negative Stain for Capsules Catalase (Reagent: Hydrogen peroxide) Coagulase (Reagent: Rabbit Plasma) Oxidase (Reagent:...
4 Photos 6. A throat swab was obtained from a paticnt with Streptococcal pharyngitis What type of expected en blood agar from the throat culture th can be either A or C 17. The species of Streptococous that causes pharymgits or "Strep throa" is IS. The pll indicator used in sugr feementation tubes is a. Neutral red b. Methyl red e. None of the above 19. Kligler's Iron agar, bocause of its composition, can be usod fo which of dhe...
Describe the genus Staphylococcus, where they can be found in/on the human body, and 3 different types of infections associated with this organism. 2. How does Staphylococcus avoid the immune response and cause disease? For each enzyme/protein or substance associated with this species note how it works. What is the best prevention for this group of organisms? 3. Staphylococcus aureus is associated with many phages that are both lytic and lysogenic. What is the difference between a lytic phage and...
3. Staphylococcus aureus is associated with many phages that are both lytic and lysogenic. What is the difference between a lytic phage and a lysogenic phage? 4.Describe the genus Streptococcus, where it is found in/on the human body, and how it isclassified. 5. What is one of the most common clinical manifestations associated with Streptococcus pyogenes? What are 3 other types of diseases associated with Streptococcus pyogenes .