Correct answer is option B i.e., 0.01 mm.
A smaller plate height is more efficient because it will lead to higher resolution of spots. If the plate is large then ability to elute will be low.
A chromatography column having which of the following plate heights would be the most efficient? a....
An analyst has to decide which of 3 chromatography columns would be the best to buy based on the column efficiency in terms of the height equivalent of the theoretical plate. Column 1: L=2.04m, TA= 9.13s, W1/2= 8.44s Column 2: L=3.00m and H = 0.01 m Column 3: L=5.00m and H = 0.03 m Calculate the height of the theoretical plate (m) for column 1
1.6 points) A tensile test specimen having a diameter of 10 mm and a gange length of 50 mm wa. tested to fracture. The stress-strain curve from the tension test is shown below. Labeling your points and showing the necessary steps, along with the corresponding calculations, find the following magnitudes: (a) Modulus of Elasticity, E = (b) Ultimate Strength = (e) Yield stress (0.2% offset method), (d) If the shear modulus of elasticity of the material is 28 GPa, determine...
question #2 please
Reverse phase chromatography is the most common type of liquid chromatography which the solid phase is made up of a packing material that is largely hydrophobic and the eluents are of varying polarities. In general, the more polar compounds elute first from the column followed by more hydrophobic compounds. PRELAB QUESTIONS 1. Draw structures for the following analytes for the simulation: a. acetophenone b. benzophenone c. butylparaben d. propiopheneone e. ethylparaben f. propylparaben g. ketoprofen h. 3-nitrophenol...
Chromatography Basics Multiple Choice: Answer the following multiple choice questions about Chromatography Basics (SHOW WORKING): 1) In a given chromatographic column, A) different solutes behave as if there are a different number of theoretical plates for each. B) the number of theoretical plates is the same for all solutes. C) efficiency is improved by increasing plate height. 2) What useful information can be found from a Van Deemter plot? A) The selectivity factor B) Optimum mobile phase flow rate C) Optimum...
ANSWER ALL 1) Which of the following is the stationary phase in size exclusion chromatography? A. resin B. mixture of proteins C. solvent or buffer D. none of these 2) Suppose you wish to purify a protein that has many positively charged residues from a mixture of proteins. Which of the following columns would elute the protein the fastest? A. column packed with neutral resin B. column packed with positively charged resin C. column packed with negatively charged resin D....
1. What is the retention factor for the following TLC plate if the baseline is 1 cm, elute solvent traveled 5cm, and the spot of interest traveled 2.5cm? a) 0.50 b) 1.6 c) 0.63 d) 1.4 10. Column chromatography employs both a stationary and a mobile phase. Which of the compounds listed below is commonly used as the stationary phase in column chromatography? a) decolonizing charcoal b) sodium bicarbonate c) benzoic acid d) silica gel
11. Why should you use pencil instead of pen when marking on a thin layer chromatography plate? a. Pencil lead isn't as dark. b. The components of pen ink will separate along with your sample, while pencil lead will not. c. Pen ink will not mark on a TLC plate. d. Pen ink will undergo a chemical reaction with the solvent. 12. Which of the following is not a reason to use thin-layer chromatography? a. To determine the number of...
The following data applies to a column for liquid chromatography: Use this information to answer the question below Length of column = 30.50 cm Flow rate = 0.313 mL/min A chromatogram of a mixture of species A, B, C, and D provided the following data: Species Retention time, minutes Width of peak base (W), minutes А 5.4 0.41 B 13.3 1.07 C 14.1 1.16 D 21.6 1.72 Calculate the resolution for species C and D Select one: O A 2.60...
Which of the following metrics would be most important to the purchasing function when the firm has an efficient supply chain strategy? average purchase quantity supply lead time supplier quality average purchase price
Put the analytes in order of increasing hydrophobicity. Which of
the compounds is most hydrophilic? Which is most hydrophobic? What
is the predicted elution order from a reverse phase chromatography
column?
a. acetophenone b. benzophenone c. butylparaben d. propiopheneone e. ethylparaben f. propylparaben g. ketoprofen h. 3-nitrophenol i. 4-nitrophenol 2. Put the analytes in order of increasing hydrophobicity. Which of the compounds is most hydrophilic? Which is most hydrophobic? What is the predicted elution order from a reverse phase chromatography...