Question

2. You've identified a mutant allele for a new gene in mice that causes muscle hypertrophy You've named the gene Mh, so the wild-type allele is Mh, while the big-muscle mutant allele you've designated as Mh89, You believe this gene is involved in muscle growth and development, but you haven't been able to isolate the protein yet. You also do not know exactly where the mutation is in the gene, but you do know that the mRNA transcript of the Mh8 mutant allele is longer In addition to having more muscle mass, you've noticed that the mutant mice always have light gray coat color, as opposed to the standard white coat of your wild-type strain. So you'd like to determine if one coat color is more likely to have one allele over another. You're going to explore this hypothesis using a Northern blot. a) Why is a Northern blot a better choice than a Western blot at this point of knowledge about the gene (1-2 sentences, 1 point) b) You've already isolated and purified RNA samples from both light gray and white mice and have your gels ready to go for electrophoresis. What else do you need to be able to perform a Northerm blot? (1 point) c) Your Northern blot results are shown below for 3 white coat mice and 6 light gray coat mice. Based on this information, do you think it's likely that different alleles for the Mh gene also contribute to coat color? What else can you determine about the relationship between the two alleles and the coat color phenotype? Explain your answer. (2-3 sentences, 1 point) coat color & individual samples white white white gray gray gray gray gray gray ladder #1 #2 #3 #1 #2 #3#4#5#6 10 kb 100 bp page 2 of 2

Answer 1

Ans:- ) Northen blot :- this is a technique that is specifically used to detect a specific type of gene or mRNA seq from the given sample

Western blot :- this is a technique that is mainly used to detect specific type of protein from a mixture of protein the method is usually based on Ag antibody reaction

Ans 2):-steps of northen blot process :-

1. RNA is extracted from the sample and separated by gel electrophoresis.
2. RNA is transferred from gel to bloting membrane and fixed
3. The membrane is treated with labeled probe prepared from cDNA or RNA .
4. The probe is then incubated with membrane so that it bindwith specific type of sequence
5. Unbound probes are washed off
6. Hybridized fragments are then detected with an autoradiograph

Ans3:-) yes it will contribute to the coat color. the relationship between two allele and phenotype depends on the dominant and the recessive trait if it is dominant than it requires only one copy of gene to appear on the body and if it is recessive then two copies are required to appear.