Question

2. Explain why electroosmotic flow predominates capillary electrophoresis and how it can affect the migration (elution) of positive, negative, and neutral species within a sample.

Answer 1

The electro-osmotic flow is generated when electrodes are placed in the reservoirs at each end of a microchannel by a process known as electro-osmosis .

Capillary electrophoresis  is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.

reason why electro osmotic flow pre dominate capillary electrophoresis is :The velocity of migration of an analyte in capillary electrophoresis will depend upon the rate of electroosmotic flow of the buffer solution.

the electroosmotic flow is directed toward the negatively charged cathode so that the buffer flows through the capillary from the source vial to the destination vial. Separated by differing electrophoretic mobilities, analytes migrate toward the electrode of opposite charge. As a result, negatively charged analytes are attracted to the positively charged anode, counter to the EOF, while positively charged analytes are attracted to the cathode.

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