Question

3. From data in the table below calculate the AG for each of the following reactions. Show all your work and calculations. (4 points) Phosphoenolpyruvate ADP Pyruvate ATP A) ATP3-Phosphoglycerate ADP 1,3-Biphosphoglycerate B) Standard Free Energies of Hydrolysis of Some Phosphorylated Compounds and Acetyl-CoA (a Thioester) AG (keal/mol) Phosphoenolpyruvate 13-Bisphosphoglycerate (3-phosphoglycerate+ P Phosphocreatine ADP (AMP+ P) ATP (ADP+P ATP (AMP+PP) -14.8 -11.8 -10.3 -7.8 -7.3 -10.9 AMP ( adenosine + P) PP,(2P Glucose 3-phosphate -3.4 -4.0 -6.0 Fructose 6-phosphate Glucose 6-phosphate Glycerol 3-phosphate Acetyl-CoA -3.8 -3.3 -2.2 -7.5 Soarce Data mostly fron lencks, W.P. (1976) anook af Bechesty and Molecular Auiogy Ted e asiman,G.0, ed), Pysical and Chemical Deta Vt. 1, 296-354,CR Press, baca Ratan IL The salue for the frer energy of hgdrolyss of is from FinyA. Aathaht A (195) Standard free-enegg change for the hyoalin of the a phosphoanngtrde bridgt in AP bachenioy 14, 11,307-11.310

Answer 1

$\Delta G$ is additive.
AG (A) = -7.5Kcal/mol
and
AG'(B) = +4.5Kcal/mol
.

Phosphoenolpyruvate + ADP Pyruneate + ATP po (P.) pyr Py8+ (P.): A6 = -14.8 kcalma! ADP + P > ATP ; 16° = +7-3 kcal/mol Ppyr + ADP Pyr + ATP ; sh = (-14.8+7-3) =- 7.5 kcalford ATP+ 3-phosphoglycerate ADP + 1,3-bis-phosphor glycerate. ATP Gly + ADP + su= – 7.3 kcal/mol. 1,3-bis (PiJ Gly; on = +11.8 kcal/mol 3- ATP + 3(Pi) Aly ADP + 1,3-bis (Pi)aly ; &h= (-7.3 +11.8) secalpes = + 4.5 kcal/mol