Question

6. How was thin-layer chromatography utilized in the aspartame experiment? (In other words, what type of information could be revealed by this technique in the experiment?)

Answer 1

1. By using TLC we can find out whether starting material consumed fully or not in other words rreactio is over or not.

2. TLC will give us idea about how many impurities formed in reaction mixture along with product.

3. TLC will give us idea about what kind of solvent we can use for column purification.

4. TLC will give us idea about impurities are very close to product or there is much Rf difference so accordingly we can run column.