Question

11. a. (12 pts) Calculate the ATP equivalents that would be produced from oxidative phosphorylation and substrate level phosphorylation if 13 lactate molecules were completely oxidized to CO2. (Enter the number of CO2 molecules that would be produced.) Give a full and clear accounting of your thought process to receive any credit. b. (3pts) What percent of the total ATP equivalents generated are from substrate level phosphorylation ?

Answer 1

Metabolic pathway of lactate 2 Lactate 2. barruvate 6ATP MADP Glucol ATP ADP Glicore-6-1 fructose - 6-P Glycolhreing 2x glyceraldehyde 3-p. ali NADT INADI 2x 1,3 bis shorphoglycorate RADP Substrate level 2ATP phophorylation 2x3 - froosho glycorate Pag LADD' ATP & Substrate level ex phosphoend fyrrvate Phorfhorylation Pyruvate 2NAOH tlh Acetyl COA } مقام پر اس 2 2 ATP ir kren 6 NADA - 2. FAD V2.CO Que to oxidation of mo lactate ATP used = 6 ATP generated 6 ATP Among 6 ATP generation 4 are due to substrate lover pronpharrylation in glyplysin b. Total NADH = 10 NADH Totol FADH = 2 FADA

INAOH = 2.5 ATP ATP oxida are phosphorylation JD NADit 2.5 X6 25 f IFADH = 15 ATP - 2 . & 15x2 =3 ATP i dueto e imolecule of lactote oxidation total, ATP eauivalent yielded & (6 + 6 et 25+ 3) ATP 2 28 ATP . I molecule Lactate yield 28 ATP earnivalent e. 13 u u u 28813 " in Total 364 ATP equivalent will be yielded. # I lactate yield 2ATP by substrate level shosphorylation 13 Lactate gielded (2x1B) = 26 ATP by substrate level phosphorylation For I molecule Lactate metabolism. 3co generated. : for 13 molecules lactate (3x13)=39. moecute, ce will be generated. 7 of substrate level ATP generation. 6 26 ADP Xoy, 304 AIP - 7. 143%