Why do different pigments migrate differently on the thin layer chromotography plate? What forces are at...
Why do different pigments migrate differently on the thin layer chromotography plate? What forces are at play?
We were using the pigments found in spinach.
Homework Answers
Thin layer chromatography depends on the separation principle.The separation relies on the relative affinity of compounds towards both the phases.The compounds in the mobile phase move over the surface of the stationary phase. The movement occurs in such a way that the compounds which have a higher affinity to the stationary phase move slowly while the other compounds travel fast.Therefore the separation of the mixture is attained.
The solvent that moves up the TLC plate by capillary action.Components in a mixture are seperated based on their abilities to bind or adsorb to the stationary phase anx their abilitiesto desorb or dissolve in the mobile solvent phase.
Add Answer to:
Why do different pigments migrate differently on the thin layer
chromotography plate? What forces are at...
Past paper question R, values for photosynthetic pigments may be determined using the technique of thin-layer...
Past paper question R, values for photosynthetic pigments may be determined using the technique of thin-layer chromatography (TLC). TLC plate Origin Solvent a. Outline what happens when spinach extract is spotted on a TLC plate and placed into a container of solvent. [2 marks] b. Explain what the Rf values represent in chromatography. [3 marks) c. State two photosynthetic pigments that could be identified using chromatography. [1 mark]
Melting Point, thin layer chromotography, and gas chromotography The recrystallization technique discusses using...
Melting Point, thin layer chromotography, and gas
chromotography
The recrystallization technique discusses using a drying agent before recrystallizing out of an organic solvent, however, we omitted the drying step before evaporating off the ethyl acetate. Since we didn't dry this sample, what potential issues might this have caused for analysis of the benzoin sample? Be specific about the effects it would have (if any) on each of the three analysis techniques we used. (4 pts)
1) Using a thin layer chromatography plate you experimentally determine the Rf values of benzhydrol and...
1) Using a thin layer chromatography plate you experimentally determine the Rf values of benzhydrol and biphenol developed with methanol as a solvent and then developed with hexane as a solvent. In methanol, the Rf of benzydrol is 0.69, and the Rf of biphenol is 0.63. In hexane, the Rf of benzhydrol is 0, and the Rf for biphenol is 0.46. Answer the following referring to the Rf values given and offer explanations based on principles of intermolecular forces. a)...
-Give a brief background on the role of pigments in photosythensis. -state your hypothesis. -finish with...
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
Classify and breifky explain the different patterns of genetic inheritance in humans Questions for the Thin...
Classify and breifky explain the different patterns of genetic
inheritance in humans
Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
Why do U.S. businesses aspire to go overseas? What are the different modes for entering an...
Why do U.S. businesses aspire to go overseas? What are the different modes for entering an international market? Can marketer play as a change agent?
What are some of the different diets that you have been on? Why do we find...
What are some of the different diets that you have been on? Why do we find ourselves on these diets? what is the diet successful and if so, how long were you able to keep the weight off?
For TLC plate separation, why do we use Rf values instead of distance measurements (e.g. the...
For TLC plate separation, why do we use Rf values instead of distance measurements (e.g. the spot travelled 5 cm from the starting line)? What would happen if we compared solvent systems using distances rather than Rf values?
What is Gold? What is the history of Gold in different cultures? Why do we still...
What is Gold? What is the history of Gold in different cultures? Why do we still use gold?
Principle of blue- white screening method(why do the colonies appear blue or white? what do we...
Principle of blue- white screening method(why do the colonies appear blue or white? what do we try to recover?what are the roles of substrate (Xgal) and the gene expression inducer(IPTG)?) this experiment was GENETIC DEFECT CORRECTION WITH BACTERIAL TRANSFORMATION In this experiment we did that first of all,1 µl of plasmid DNA was added into the tube which contains competent cells and the tube was tapped gently to mix DNA and the competent bacteria.After that it was placed on ice...
Past paper question R, values for photosynthetic pigments may be determined using the technique of thin-layer chromatography (TLC). TLC plate Origin Solvent a. Outline what happens when spinach extract is spotted on a TLC plate and placed into a container of solvent. [2 marks] b. Explain what the Rf values represent in chromatography. [3 marks) c. State two photosynthetic pigments that could be identified using chromatography. [1 mark]
Melting Point, thin layer chromotography, and gas
chromotography
The recrystallization technique discusses using a drying agent before recrystallizing out of an organic solvent, however, we omitted the drying step before evaporating off the ethyl acetate. Since we didn't dry this sample, what potential issues might this have caused for analysis of the benzoin sample? Be specific about the effects it would have (if any) on each of the three analysis techniques we used. (4 pts)
-Give a brief background on the role of pigments in
photosythensis.
-state your hypothesis.
-finish with stating the rationale for your hypothesis (how
did you arrive at these hypothesis?)
this is an introduction piece, so it doesn't need the results to
write the papee. introduction sections set up the background,
purpose, rationale, and hypothesis for a study.
Biod 20 PHOTOSYNTHESIS Background Plants harness the energy of the sun through a process called photosynthesis. The leaf is the plant organ that...
Classify and breifky explain the different patterns of genetic
inheritance in humans
Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago