Question

Detection of Sickle-Cell Disease by Gel Electrophoresis Firee samples of hemoglobin have been subjected to protein gel electrophoresis. Protein gel electrophoresis different composition. ) is carried out in the same manner as DNA gel electrophoresis (see Fig. 10.8) except the gel hasa 1. Sickle-cell hemoglobin (Hb) migrates more Figure 10.10 Gel slowly toward the positive pole than normal hemoglobin (Hb) because the amino acid valine has no polar R groups, whereas the amino acid +Pole glutamic acid does have a polar R group. 2. In Figure 10.10, which lane contains only Hb signifying that the individual is Hb Hb 3. Which lane contains only Hb, signifying that the individual is HVHp? 4. Which lane contains both Hbs and Hb, signifying -Pole that the individual is Hb Hb? Lane 1 Lane 2 Lane 3 Detection by Genomic Sequencing You are a genetic counselor. A young couple seeks your advice because sickle-cell disease occurs among the family members of each. You order DNA base sequencing to be done. The results come back that at one of the loci for normal hemoglobin, each has the abnormal sequence CAC instead of CTC. The other locus is normal. What are the chances that this couple will have a child with sickle-cell disease? Conclusion: Detecting Genetic Disorders What two methods of detecting sickle-cell disease were described in this section? Which method is more direct and probably requires more expensive equipment to do?_ Which method probably preceded the other method as a means to detect sickle-cell disease?

Answer 1