Question

Prussian blue stain is used to evaluate iron in the bone marrow. What precautions must be taken with the sample and or stain?

Answer 1

ANS)

The perls prussian blue is normally utilized on bone marrow aspirates to specify the range of iron cache and will give dependable proof of iron insufficiency.

The precautions that should be taken with sample and stain are,

1) Well known and recognized positive control segment should be utilized to guarantee accurate differentiation have beeen attained.

2) The Neutral buffered formalin provides the accurate outcomes.Another fixatives can be utilized,even so acidic fixatives,acidic decalcification solutions and dichromate fixativesmust be evaded.These type of mixtures will leads to continuing hydrolytic mislaying of ferric ions from the group of cells( Tissues) and a opposing outcome should be considered with intution.

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