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A binding protein binds to a ligand with Kd=30nm. What is the concentration of ligand when...

A binding protein binds to a ligand with Kd=30nm. What is the concentration of ligand when the [L] when the fraction bound,\theta, is a) 0.25, b) 0.6 and c) 0.95.

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Answer #1

Q = [L] Kd+ [1] o Kd fraction bounded constant 1) 0 = 0.25 0.95 - (L] 30+ [4] [L] - 0.25 X 30 + 0.251 (.] (1-0.25) = 0.25x30

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