Question

DNA containing broken phosphodiester bonds ("nicks") can be repaired by the action of a ligase enzyme. Formation of a new phosphodiester bond in DNA requires the free energy of ATP, which is hydrolyzed to AMP: ligase ATP + nicked bond - AMP + PP, + phosphodiester bond The equilibrium constant expression for this reaction can be rearranged to define a constant, C, as follows: K [phosphodiester bond][AMPUPP1 [nick][ATP] [nick] [AMP][PP:1 [phosphodiester bond] Keq[ATP] [PP1 Keq[ATP] [nick] = C[AMP) [phosphodiester bond] Studies show that the value for C is 1.8x10-3. As such, when the concentrations of PP; and ATP are 1.0 mM and 14 uM, respectively, the value for keg is __ significant figures and scientific notation.] [Express your answer using 2 x 10 What is the value of AGⓇ' for the reaction in units of kJ/mol? [Express your answer using 2 significant figures.] kJ/mol the tolerance is +/-2%

Answer 1

CPP ker CATP) CPPi Kea C [ATP) (roXI3) .8x163 u.ox10 RTM KeR ব७°- 8-314X8 (4.oX10) 1- 26 kmo