Homework Answers
1. Formula :
Particle per microliter volume = counted particles / counted surface × chamber depth × dilution
WBC :
Counted number of WBC = 6
Counted surface = 4mm2
Chamber depth = 1/ 10mm
Dilution = 1:20
= 6× 50 =300 cells /microliter volume is the number of WBC particles
2. Formula to Cala the number of RBC :
Number of cells counted × dilution factor × 5 / volume ( 0.1 )
= Number of RBC counted -12
Dilution factor -1:20
= 12× 1× 5/ 0.1 ×20
= 12000 cells /microliter volume
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