Question

to differentiate the örgall Characteristic used to differentiate organisms Organísms Media used Salmonella vs. Shigella Enterobacter vs. Escherichia Klebsiela vs. Enterobacter Klebsiella vs. Shigella Proteus vs, Citrobacter

Answer 1

Answer :

Salmonella vs shigella :

Salmonella : colorless, usually with black center

Shigella : colorless .

• All members of shigella are aerobic and facultative anerobesand gram negative bacteria .
• Grow readily in culture media at PH 6.4 to 7.8 at 10 oC to 40 0C ,with optimum temperature 37 oC
• The colonies are circular ,convex ,colorless but moderately translucent with smooth surface and entire edges.
• Small tangled hair like projections can sometimes be seen at one or more points on the periphery of the colony .
• Non motile .
• Do not produce H2s
• No gas from glucose fermentation.
• Medium used for shigella is XLD agar .

Salmonella :

Gram stain : gram negative .

Motility : motile .

Catalase : positive .

Oxidase : negative .

Medium used for : choose lysine deoxycholate is a selective growth medium .

2) enterobactor vs Escherichia :

Escherichia coli :

• Gram negative motile rods .
• Non spore forming ,facultative anaerobic ,oxidase - 've
• Ferment glucose and lactose .
• Normal flora of intestine .
• Medium used for : coliform chromoselect agar is a selective chromogenic medium recommended for simultaneous detection of Escherichia coli.

Enterobactor :

• Gram negative .
• Facultative anaerobic ,rod shaped ,non spore forming bacteria .
• Oxidase negative .
• Catalase positive
• Insole negative .
• Medium used for :coliform chromosome select agar .

3) klebsiella :

Non motile

Simmons citrate positive

H2s negative ,weakly urease positive.

MR negative ,Vp positive .

Medium : hi chrome klebsiella selective agar base is used for the selective isolation and easy detection of klebsiella species from.water and other sources.

4) proteus vs citrobactor :

Proteus :

• Gram negative .
• Facultative anaerobic ,rod shaped bacterium
• It shows swarming motility and urease activity
• Medium used for proteus : a medium.containing heart infusion agar supplemented with bile salts ,lithium chloride ,sodium thiosulfate and sodium citrate was developed for selective growth of proteus.

Citrobactor :

• Gram negative .
• Motile .
• Rod shaped
• Grow in ordinary media , colonies on nutrient agar