Question

In reverse-phase HPLC, the liquid stationary phase is _____ polar than the mobile phase, and elution of the ______ polar compounds occurs first, with the ______ polar compounds adsorbed more tightly to the stationary phase.

Select one:

a. less, less, less

b. more, more, more

c. less, more, less

Answer 1

In reverse - phase HPLC , the liquid stationary phase is more polar than the mobile phase , and the elution of the more polar compound occur first , with the more polar compounds absorbed more tightly to the stationary phase