Question

3. Explain with the example of a hypothetical molecule (R.S)-A the concept of separating enantiomers with a chiral resolving agent (R)-B

Answer 1

Example for a hypothatical molecules: - = > (R, s) - A is racemic mixture that contains the enantiomers of compound A (R) - B is a pure chiral compound containing a single enantiomer = > (R, s) - A reacts and form a (R - B) derivatives These derivatives have diastereomeric relationships each other \r\n = > (R, R) - AB and (s, R) - AB are diastereomers They have diff physical and chemical properties. They can differ in solubility in certain solvents. = > The way of separating distereomer from the other by exploiting their solubility difference. = > By forming crystals then can differ. one may crystalize out and other may remain in solution They will have different retention time in HPLC = > This type of separating is called column chromatography = > By using above techniques we can separate the diastereomers = > After the separation the derivatives can chemically broken down to their starting components. = > The racemic mixture can be resolved into pure enantiomers