Question

Please completely answer the below biochemistry question based on the provided Figure. Please show/explain all work.

3. The authors of this study studied the ability of N-1,5-dimethylhexylformamide to inhibit mouse ADH1. The activity of the enzyme was measured in the absence of inhibitor, and in the presence of 1.0 μM inhibitor. The data are presented in Table 13.1. Table 13.1: Inhibition of mouse ADH1 by N,1-5-dimethylhexylformamide ADHI velocity, Δ NADH | ADHI velocity, Δ NADH absorbance/min (with inhibitor) Ethanol Concentration, mM|absorbance/min (without 0.20 0.25 0.36 0.60 2.00 inhibitor) 0.036 0.042 0.048 0.065 0.075 0.022 0.024 0.027 0.029 0.033

Answer 1

y = 3.3062x + 28.568 Vo Vo 1/VO 1/10 (without (with (without (with [S] inhibitor) inhibitor) 1/[S] inhibitor) inhibitor) 0.21 0.036 0.022 5 27.77778 45.45455 0.25 0.042 0.024 4 23.80952 41.66667 0.048 0.027 2.777778 20.83333 37.03704 0.6 0.065 0.029 1.666667 15.38462 34.48276 0.075 0.033 0.5 13.33333 30.30303 y = 3.2861x + 11.063 1/Vo 1/Vo (without inhibitor) 1/VO (with inhibitor) Without inhibitor y = 3.2861x+11.063 1/Vmax = 11.063 Vmax = 0.0904 absorbance / min Km = 3.2861*Vmax Km = 3.2861*0.0904 = 0.2971 mm 1/[S] Calculation of K Calculation of a 1+0) a=1+[1] K K a=1+ 0.001 With inhibitor y=3.3062x+28.568 1/Vmax = 28.568 Vmax = 0.035 absorbance / min Km = 3.3062*Vmax Km = 3.3062*0.035 = 0.1157 mm [I]=1 uM = 0.001 mM 1 0.2971 0.1157 = -0.001 0.000638 a = 2.567 1+- K = 0.000638 mM