Question

3)TA student needs to determine the concentration of Tryptophan (Try) amino acid that absorbs UV light at 282 nm. Absorbance of Tryptophan in an unknown analyte sample previously diluted 1:8 was 0.673 at 282 nm. Calibration of UV-Vis spectrometer was performed by serial dilution of a Tryptophan standard (230 ppm). A student diluted standard in the following ratios: 1:10, 1:20, 1:50, 1:60 and 1:70 and determined Absorbances of 1.923, 0.942, 0.443, 0.367 and 0.294 respectively. What is the concentration of Tryptophan in an unknown analyte? Determine calibration equation and correlation coefficient value.