Question

Problems on Gel-Permeation Chromatography (GPC) 1. A gel-filteration column has a radius (r) of 0.80 cm and a length of 20.0 cm. (a) Calculate the total volume of the column (b) Blue Dextran ( a protein) was eluted in a volume of 18.2 mL. What volume is occupied by the stationary phase plus the solvent inside the pores of the stationary phase? (c) Suppose that the pores occupy 60% of the stationary-phase volume. Over what volume range (x mL for the largest molecules to y mL for the smallest molecules) are all solutes expected to be eluted?

Answer 1

Answer 1):

a): Total volume of column= pi×r^2×l=3.14×(0.80^2)×(20.0) cm^3= 40.2 cm^3= 40.2 ml

b): Volume is occupied by the stationary phase plus the solvent inside the pores of the stationary phase= 18.2 ml + 40.2 ml= 58.4 ml

c): Volume (pores)= (40.2 ml× 60/100)=24.1 ml

Hence, volume range is 0-24.1 ml

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