Question

biochemistry. please help and check my work. please show work

4. What is the yield in molecules of ATP when one molecule of each of the following substances is completely oxidized by a cell to CO2 and H20. Assume that glycolysis, the citric acid cycle, malate-aspartate shuttle, beta- oxidation, electron transport chain, etc., are all fully active. Briefly show where your numbers are from INADH 3 a. pyruvate 3 NADH IFADHz 2 16 тр 15 129 5 b. Palmitic acid (16:0) то те 12 Атр qu+ 21 +14-2 -129 : x12 376 NADH 21:7, ZIAT 17. ATP214 FARHZ 223 c. Fructose-1,6-diphosphate NADH G ZATP 2 INADH 3 3 NADH 9 IFADH₂2 OTP I Z3

Answer 1

a)

From pyruvate to completion of one round of Krebs cycle, four NADH (Once is during pyruvate to acetyl coA conversion and Three during Krebs cycle) and one FADH2 is produced. and 1 ATP (from GTP) so total ATPs are 12.5 .

b)

Oxidation of palmitic acid yields 7 NADH + 7 FADH2 + 8 acetyl-CoA in 7 cycles of mitochondrial beta-oxidation. Every acetyl-CoA yields 3 NADH + 1 FADH2 + 1 GTP (=ATP) during Krebs cycle. An average production of 3 ATP/NADH and 2 ATP/FADH2 using the respiratory chain, We get 131 ATP molecules

c)

It should convert into glucose which yields 2 ATP molecules. Then this glucose enters into glycolysis and citric acid cycle which yields 38 ATP molecules. So, altogether 40 molecules of ATP are synthesised if fructose 1,6 diphosphate undergoes respiratory cycles.