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10. a) Tranamittance (T) = I/Io =
I = intensity of the light after passing through sample. I0 = intensity of the incident light.
T = 0.025/0.5 = 0.05
B) absorbance A = - log T = - log 0.05 = 1.30
C) when stray light is used
Incident intensity and intensity after absorption both changes
SL is intensity of stray light.
Absorbance (A) = -log (I + SL)/(I0+ SL)
= -log ( 0.025+0.010/0.5+0.010)
= - log( 0.035/0.51) = 1.163
Perecentage error = |(measured - accepted)| *100/ accepted
( 1.30-1.163)*100/1.30 = 10.53 %
10. (8 pt) The light intensity from the reference cell in a dual-beam spectrometer (Po) is...
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