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chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
5. When all of the sample to be collected has left the
column, will the drop of solution coming off the tip of the column
be acidic, basic or neutral?
6. How many H+ ions would be displaced from the ion
exchange column by 1 Cu2+ ion?
7. Binding sites on the ion exchange resin are saturated
with which ion before the sample solution is added?
8. Ions are displaced from the resin binding sites by
any ion that has...