Question

Anx 628 nm € (R.PM cm") ECM'cm') (B, um cm) Absorbanco 0.000095 0 0.0434 0.57 0.0246 0.000823 0.000714 0.31 501 nm 427 nm 0.00759 0.0251 0.0027 0.57 With the blue dye concentration determined to be 13 pm, what is the next step in determining the concentrations of the other dyes? Click the answer you think is right. There is no other step. The blue dye concentration is the only one that can be determined in this mixture. Ignore the absorbance of the blue dye at 501 nm and 427 nm, and solve the equations 2 and 3 for the concentrations of the red and blue dyes. Adjust the absorbances at 427 nm and 501 nm by subtracting the blue absorbance at these wavelengths.