Question

In a FRET experiment, if a donor-acceptor distance is 12% longer than the Förster distance, what is the efficiency of FRET expressed as a fraction? Respond with the correct number of significant figures in scientific notation (Use E notation and only 1 digit before decimal e.g. 2.5E5 for 2.5 x 10") Numeric Answer:

Answer 1

The FRET efficiency is given by the equation,

E = R6 + R6 R6 + b

Where, R is the distance between donor and acceptor pair.

R₀ being the Förster distance.

Förster distance is the distance between donor and acceptor at which the FRET efficiency is 50%.

It is given that R is 12% longer than the R₀, Förster distance.

That is R = R₀ + 0.12R₀

= 1.12 R₀

_{substituting these values in our equation, we get the FRET efficiency as:}

  
_{ROS E RE + + [ +0.1279] j 1+(1.12) = 0.336 = 3.36E(-1) I+1.9738}