Question

Explain the mechanism using the R as PMB and the reagents in 11B

References Mailings Review View Help Tll me what you want to do Paragraph ZHN CO2Me Thiol ZHNCO2Me deprotection 3 SH 12 11a-c 11 b, R PMB (8796) Hg(OAc)2 (2eq). DTT(2 eq.) / TFA (75%)

Answer 1

IN thiol deprotection hydride ion acts as nucleophile and relaces R. Understand tge logic.Thiol-modified oligonucleotides have a number of uses, including reaction with labels that contain an α,β-unsaturated ketone, maleimide or other Michael acceptor. They can also be used for reaction with cysteines in proteins to made disulfide bonds and for binding to gold nanoparticles. For further information on uses of thiol-modified oligos see 5′-Thiol oliognucleotides and alkaline phosphatase probes.