Question

You are a graduate student working in the bioengineering lab. Within the scope of your project, the laboratory director asks you to produce biodiesel with waste cooking oil and methanol using the lipase of a microorganism that produces intracellular lipase enzyme. You know that lipase production will be on a laboratory scale and biodiesel production will be in the bench top reactor. Starting from the production of lipase, draw a flow chart including biodiesel production and briefly explain each stage.

Answer 1

wateor, Biodiesel Transestrefilation and estar process Process Coletween from the above to in the bottom block will have Sowinni- The procesy Cos of generating Bio diesel from waste methand having follow steps: oil and cole Cookings poret orcat munt process:- in this procorx Whaleveo Dil we have taklu forem that oil all impuities will be removed. The cleaned via titaation against Cheik in the Bio-diesel. Pae mining processi oil will be tested standard solution to concentration of fatty acids present Rooking oil Note:- this fatty acids shoud be esterified to produce Enzymatic reaction:- In this lipase enzyme aut as aut as Catalyst quids into And loweat Gily ceaines, methanol fatty Corude oil collation : components generated will be isolated heele In the top block Conde diesel and Glycerides a futy audas a fatty acids will get stered. card and water Guy as two blocks GI CSScanned with CamScanner

Post tallatment process.- here the Caude Bio diesel will be Purified and results Pure Bio diesel. Flow Chart will be as below:- Cooking oil oin Enzymatic reaction (presence of Catalyst premixing Pretreatment process Process (ory enzyme Caude oil Colection Coude biodiesel tacids Coude Biodiesel Glycerol+H2O Post treatment Porcess Grycerol Collector Pure Bio Diesel S$canned with CamScanner