Question

Bicino ( Hi Na). Mm 163.2 g/mol, pka 28.85 as the Carmen Common name for one of the so-called "Lood's buffungss". ubiquitous in biochemical applications. How mang grung og bicine (the acidic form, most bu added to alloo.omh lo a a Solution, whose Concestration is o.255 m in the angugute buru form to prepare a pH = 8.08 buffer?

Answer 1

we shall use the Hendersson equation to solve this problem PH :pkat log [salt] Tacida Here, [& alt] is one of coniugate base of Bicine 0. 255(M) [acid] is cone of bicine - I Bicine] :: 8.08 : 8.35 + log 0.255 07. -0.27 و ما [Bilim 0.255 (Bicine] = 0.27 or; dog [ Bicine 0.255 [Bicine] : 0.29 0.255 x 10 -0.475 (M) I hus ; in 400 ml, moles of Biline present = O.KAS 400 10 0.1811 mole 3.2 g : Amount of Bicine required =10.1899 x 163. 30.999 31 g (A) Hope it is helpful.