Below are the Kcat and Km values for 5 hypothetical enzymes. Which enzyme would have the...
Below are the Kcat and Km values for 5 hypothetical enzymes. Which enzyme would have the largest specificity constant?
Enzyme A - Kcat = 2.6 x 103 , Km = 4 x 10-4
Enzyme B - Kcat = 9 x 102 , Km = 6.1 x 10-6
Enzyme C - Kcat = 1.7 x 10-3 , Km = 3.2 x 105
Enzyme D - Kcat = 2.6 x 10-7 , Km = 4 x 104
Enzyme E - Kcat = 800, Km = 0.03
Homework Answers
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Below are the Kcat and Km values for 5 hypothetical enzymes.
Which enzyme would have the...
please help!! please please "Looking at table 6-8 on Enzyme Reactions from the lecture, which achieves...
please help!! please please
"Looking at table 6-8 on Enzyme Reactions from the lecture,
which achieves an excellent specificity constant with a very
favorable kcat (insert answer here) , which with a
favorable Km (insert answer here) , and which with
moderate values for each (insert answer here) ?
(pick from acetyl cholinesterase, B-lactamase, and catalase)"
Question 2: "The units of Vmax are (insert answer
here) and the units of Km are (insert answer
here) (pick from M, M-1, M/s, s/M...
Values for an Enzyme and a substrate are: Km=4 uM and kcat=20/min. For an experiment where...
Values for an Enzyme and a substrate are: Km=4 uM and kcat=20/min. For an experiment where [S]=6mM, it was found that Vo=480nM/min. What was the enzyme concentration? Give your answer in nM. Using the same kcat and Km as the previous question, if [Et]=0.5uM gives a Vo=5 uM/min, what wat the [S]? Give your answer in uM. reaction is run with the kcat=20/min and the Km=4uM. Use the enzyme concentration from question 1 above. A very strong inhibitor is added creating...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound most tightly by his engineered enzyme. Which value should he measure and pay attention to? Km KCAT Vo Both A and B kcat, [S], and [E]t Rodrigo's enzyme was assayed with three substrates (A, B, and C). The SAME enzyme concentration used was for each of the three reactions. The results of the three experiments are plotted as Lineweaver-Burke plots below. Considering what you...
9. What is the volume of a kilogram of water? - 10. What is the weight...
9. What is the volume of a kilogram of water? - 10. What is the weight of five liters of water?_tx1000R o 11. Complete the following calculations. Give your answers in scientific notation: 1.0 X 106* 1.0 X 103 = 7.2 x 10-4 * 6.1 x 10-7 = 4.1 X 103 * 2.9 X 10-5 = 8.4 X 106/2.1 x 104 = 1.0 X 105/3.3 x 10-8 = 6.1 x 10-3/3.2 x 10-4 = [10** 10- ) *[104 * 10-s)....
Refer to the below Enzyme activity data (Table 4) to determine the following: (1.5 pts each) a) V...
Refer to the below Enzyme activity data (Table 4) to determine the following: (1.5 pts each) a) Vmax b) why is the velocity v constant at [S] greater than 2 x 10-3 M? c) what is the free [E] at [S] = 2 x 10-2 M? 2. SI (mol/L 2 x 10-l 2 x 10-2 2 x 10-3 2 x 104 5 x 10-4 1,3 x 10-5 ol/min 60.00 60.00 60.00 48.00 45.00 2.00 Table 4. Enzyme activity assay data...
4. For which of the following 0.010 M diprotic acids would the second dissociation affect the...
4. For which of the following 0.010 M diprotic acids would the second dissociation affect the pH significantly? Explain your answer. H2A; Kai = 4.2 x 102, K2 = 1.8 x 10-7 b. H2B; Kai = 2.4 x 104, K2 = 6.1 x 108 H2C; Kai = 1.3 x 104, K2 = 5.2 x 10-9 H2D; Kai = 1.8 x 10", K 2 = 9.3 x 104 c.
Ks is the dissociation constant Note: Numbers without units are often meaningless. 1. The maximum possible...
Ks is the dissociation
constant
Note: Numbers without units are often meaningless. 1. The maximum possible rate for a reaction between two molecules occurs when every collision results in reaction. In this case the rate is predicted to be k = (472) (D2+D2)r12 with units of Mi's! Here N is Avagadro's number (6.023 x 1023 molecules/mole), r12 is the critical distance for reaction, and D, and D2 are the diffusion coefficients for the two participants. In a typical enzyme catalyzed...
Which mutation below is the least detrimental to the enzyme function? pH 8.0 Km (AM) 15...
Which mutation below is the least detrimental to the enzyme function? pH 8.0 Km (AM) 15 17 20 13 21 41 variant wild-type H57A H57L H57D H57E H57K HSOR D102N H57A, D102N H57D, D102N HSOK, D102N H57L, D102N S195A S195T H57A, D102N, S195A kbuffer (min) no enzyme kost (min-') 3200 0.054 0.075 0.78 0.69 0.83 0.017 1.3 0.17 0.18 0.41 0.13 0.079 0.011 0.038 67 keaK 210 0.0032 0.0037 0.06 0.033 0.020 0.00025 0.30 0.0019 0.0029 0.023 0.0031 0.0019 0.00052...
9) The equilibrium constant is given for one of the reactions below. Determine the value of...
9) The equilibrium constant is given for one of the reactions below. Determine the value of the missing equilibrium constant. 104 H2(g) + Br2(8) = 2 HBr(g) 2 HBr(g) = H2(g) + Bra(8) Ke3.8 Ke=? A) 1.9 x 104 B) 5.3 x 10-5 C) 2.6 x 10-5 D) 6.4 x 10-4 E) 1.6 % 103 Answer: C Diff:2 Var: 1 Global: G4 LO: 14.3 Page Ref: 14.3
a. what are the values of Vmax and Km in the abscence if the inhibitor what...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
please help!! please please
"Looking at table 6-8 on Enzyme Reactions from the lecture,
which achieves an excellent specificity constant with a very
favorable kcat (insert answer here) , which with a
favorable Km (insert answer here) , and which with
moderate values for each (insert answer here) ?
(pick from acetyl cholinesterase, B-lactamase, and catalase)"
Question 2: "The units of Vmax are (insert answer
here) and the units of Km are (insert answer
here) (pick from M, M-1, M/s, s/M...
Rodrigo is an enzyme engineer. He wants to know which of four peptide substrates is bound most tightly by his engineered enzyme. Which value should he measure and pay attention to? Km KCAT Vo Both A and B kcat, [S], and [E]t Rodrigo's enzyme was assayed with three substrates (A, B, and C). The SAME enzyme concentration used was for each of the three reactions. The results of the three experiments are plotted as Lineweaver-Burke plots below. Considering what you...
9. What is the volume of a kilogram of water? - 10. What is the weight of five liters of water?_tx1000R o 11. Complete the following calculations. Give your answers in scientific notation: 1.0 X 106* 1.0 X 103 = 7.2 x 10-4 * 6.1 x 10-7 = 4.1 X 103 * 2.9 X 10-5 = 8.4 X 106/2.1 x 104 = 1.0 X 105/3.3 x 10-8 = 6.1 x 10-3/3.2 x 10-4 = [10** 10- ) *[104 * 10-s)....
Refer to the below Enzyme activity data (Table 4) to determine the following: (1.5 pts each) a) Vmax b) why is the velocity v constant at [S] greater than 2 x 10-3 M? c) what is the free [E] at [S] = 2 x 10-2 M? 2. SI (mol/L 2 x 10-l 2 x 10-2 2 x 10-3 2 x 104 5 x 10-4 1,3 x 10-5 ol/min 60.00 60.00 60.00 48.00 45.00 2.00 Table 4. Enzyme activity assay data...
4. For which of the following 0.010 M diprotic acids would the second dissociation affect the pH significantly? Explain your answer. H2A; Kai = 4.2 x 102, K2 = 1.8 x 10-7 b. H2B; Kai = 2.4 x 104, K2 = 6.1 x 108 H2C; Kai = 1.3 x 104, K2 = 5.2 x 10-9 H2D; Kai = 1.8 x 10", K 2 = 9.3 x 104 c.
Ks is the dissociation
constant
Note: Numbers without units are often meaningless. 1. The maximum possible rate for a reaction between two molecules occurs when every collision results in reaction. In this case the rate is predicted to be k = (472) (D2+D2)r12 with units of Mi's! Here N is Avagadro's number (6.023 x 1023 molecules/mole), r12 is the critical distance for reaction, and D, and D2 are the diffusion coefficients for the two participants. In a typical enzyme catalyzed...
Which mutation below is the least detrimental to the enzyme function? pH 8.0 Km (AM) 15 17 20 13 21 41 variant wild-type H57A H57L H57D H57E H57K HSOR D102N H57A, D102N H57D, D102N HSOK, D102N H57L, D102N S195A S195T H57A, D102N, S195A kbuffer (min) no enzyme kost (min-') 3200 0.054 0.075 0.78 0.69 0.83 0.017 1.3 0.17 0.18 0.41 0.13 0.079 0.011 0.038 67 keaK 210 0.0032 0.0037 0.06 0.033 0.020 0.00025 0.30 0.0019 0.0029 0.023 0.0031 0.0019 0.00052...
9) The equilibrium constant is given for one of the reactions below. Determine the value of the missing equilibrium constant. 104 H2(g) + Br2(8) = 2 HBr(g) 2 HBr(g) = H2(g) + Bra(8) Ke3.8 Ke=? A) 1.9 x 104 B) 5.3 x 10-5 C) 2.6 x 10-5 D) 6.4 x 10-4 E) 1.6 % 103 Answer: C Diff:2 Var: 1 Global: G4 LO: 14.3 Page Ref: 14.3
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
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