Question

1) What pipetman would you use to drspen 600ul? 17ul? 70ul? 2) What size pipet (1, 5, 10, 25ml) would you use to dispense: 2ml? 8ml? 20ml? 3) Convert: a. 5 x 10 ml to ul b. 7 ml to ul c. 50 g/1 to mg/1 d. 0.4 mg/1 to mg/ml e. 75 mg/ml to ug/ul f. 10 M to mM g. 10 M to uM 4) Divide 2.5 x 107 by 103 5) Multiply 5.2 x 107 by 103 6) How many grams of dextrose needs to be added to what volume of water in order to make a 100 ml of a 5% dextrose solution in water? (1 g solid= 1 ml water) 7) What volume of a 40% dextrose solution needs to be mixed with what volume of water in order to make 1 liter of a 2% dextrose solution? 8) How many moles and grams of NaOH (MW 40.0) need to be added to 500 ml of water to make a 7 M NaOH solution? ) Dilute a 8 M NaCl solution 1:49 to a final volume of 100 ml. What volume of 8 M NaC eeds to be added to what volume of water?

Answer 1

Q.1. Not visible clearly

Q.2. Answer: 20 ml

We can use 20 ml Pipet three times to dispense 1,5,10 and 25 ml

Q.3.

Answer A. 5X10^-2 = 50 µL

B. 7 ml = 7000 µL

C. 50 g/L = 50 mg/ml

D. 0.4mg/L = 0.0004 mg/ml

E. 75 mg/ml = 75 µg/ µL

F. 10 M = 10,000 mm

G. 10 M = 10000000 µm

Q.4. Divide 2. 5 X 10 ^-7 / 10 ^-3

Answer: 0.00025

Q.5 Multiply 5.2 x 10 ^-7 / 10 ^-3

Answer: 5.2e-10

Q.6.

Answer: Dissolve 5 grams of dextrose in 100 ml of water, it gives 5% of 100 ml of dextrose solution

Q.7.

We required 0.05 liters of 40% dextrose to prepare 2% of 1 liter dextrose

Q.8. To prepare 500 ml 7 M sodium hydroxide, we need to dissolve 140 grams

Q.9.

Answer: 1 ml of 8 M Nacl to dilute with 49 ml of water

Q.10.

Answer: To prepare 100 ml 0.5 M NaOh solution, dissolve 2 grams in 100 ml of water

Q. 11.

Answer: to prepare 2M NaCl and 0.5 M NaoH, dissolve 58.44 g of NaCl and 2 grams of NaoH in 500 ml of water

Q.12

To prepare 10% SDS, dissolve 10 grams of SDS in 100 ml of water

Q. 13.

To prepare 0.1 M EDTA/ 0.2 M Tris/ 0.4 M NaCl, dilute 100 ml of 0.5 M EDTA/ 100 ml of 1M tris and 200 ml of 1M NaCl