Question

Post Lab Questions: CI MALACHITE GREEN CRYSTAL VIOLET 1. Why does the malachite green dye elute first? What physical properties does it have that affect it's interaction with alumina and how are those different from crystal violet? 2. Write the reaction of crystal violet with 1 equivalent of HC. 3. Why does adding HCI cause crystal violet to elute more quickly? What changes with it's physical properties and how does that property affect it's interaction with alumina?

Answer 1

Here you go:

As we can see malachite green looks in one group from crystal violet. Thus, malachite green is less polar compound to the crystal violet. This is the reason why malachite green will emit fast. Now, since alumina is the stationary phase, that means the stationary phase is polar one and it will interact more with a polar compound. In between the two dye, we see that crystal violet is more polar, thus it will interact more with alumina and thus slower compound to malachite green.

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