Question

2. Will a 50 mM solution of HEPES at a pH of 8.0 be a better buffer towards OH or H addition? Discuss. The pKa of HEPES is 7.6.

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Answer #1

The pKa of HEPES is 7.6

The pH for the best buffer will be 7.6. The solution is at pH = 8.

So; the pH should decrease to show buffer activity. You have to add H+ to reduce the pH.

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