Question

[S]             v

(nM)      (nM s-1)

1.25          1.10

3.60          4.15

5.83         6.54

7.34        9.38

9.12        11.71

Calculate the Vmax and Km values using the following methods with work.

Line weaver Burke:

Hanes Woolf:

Eadie Hofstee:

Answer 1

Lineweaver Burk:

Equation:

1 V Km 1 SV mar' Km

S	V	1/S	1/V
1.25	1.1	0.8	0.909091
3.6	4.15	0.277778	0.240964
5.83	6.54	0.171527	0.152905
7.34	9.38	0.13624	0.10661
9.12	11.71	0.109649	0.085397

Lineweaver Burk Plot = 1.203x0.061 R=0.997 NT TIITTO 0.2 0.4 0.6 0.8 1/[S]

Comparing with the equation in the plot the standard equation

1/Km = 0.061

=> Km =16.39

Km/Vmax = 1.203

=> Vmax=16.39/1.203

=> Vmax = 13.62 nM/s

Hanes Woolf:

Equation

(s) _ Is Km V Vmar. Vmar

V	S	S/V
1.1	1.25	1.136364
4.15	3.6	0.86747
6.54	5.83	0.891437
9.38	7.34	0.782516
11.71	9.12	0.778822

Hanes Woolf Plot y=-0.041x1118 R = 0.789 NIS) O 2 4 6 8 10 [s]

Comparing the equation in the plot with the standard equation

1/Vmax=0.041

=>Vmax= 24.39 nM/s

Km/Vmax = 1.118

=> Km = 24.39*1.118

=>Km=27.268

Eadie Hofstee:

Equation:

V = -Km * V - + V mar 1s

S	V/S	V
1.25	0.88	1.1
3.6	1.152778	4.15
5.83	1.121784	6.54
7.34	1.277929	9.38
9.12	1.283991	11.71

Eadie Hofstee Plot y= 23.37x20.14 R2 = 0.840 0 0.2 0.4 0.6 1 1.2 1.4 0.8 v/[S]

Comparing with the equation in the plot the standard equation

Vmax= 20.14 nM/s

Km= 23.37

Summary of all answer:

	Lineweaver Burk	Hanes Woolf	Eadie Hofstee
Vmax	13.62	24.39	20.14
Km	16.39	27.268	23.37